Comparative Study of Commercial Nucleic Acid Extraction Kits for Detecting Microorganism
نویسندگان
چکیده
منابع مشابه
Comparative evaluation of three commercial systems for nucleic acid extraction from urine specimens.
A nucleic acid extraction system that can handle small numbers of specimens with a short test turnaround time and short hands-on time is desirable for emergent testing. We performed a comparative validation on three systems: the MagNA Pure compact system (Compact), the NucliSens miniMAG extraction instrument (miniMAG), and the BioRobot EZ1 system (EZ1). A total of 75 urine specimens submitted f...
متن کاملComparative Study of Extracted Nucleic Acid from Escherichia coli by Two Methods Phenol-chloroform and Extraction Using Magnetic Nanoparticle
Background: The most important researches in molecular and genetic engineering fields are the finding of optimal methods for extraction of the genomic content of microorganisms and cells using them, can achieve the most amount and purity with the least time and cost. There are several methods for the extraction of bacterial DNA and the use of magnetic nanoparticles is one of the novel methods o...
متن کاملMolecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
Molecular beacons (MBs) of DNA and RNA have aroused increasing interest because they allow a continuous readout, excellent spatial and temporal resolution to observe in real time. This kind of dual-labeled oligonucleotide probes can differentiate between bound and unbound DNA/RNA in homogenous hybridization with a high signal-to-background ratio in living cells. This review briefly summarizes t...
متن کامل8.5 Detecting Nucleic Acid Hybridization
The double-helical structure of nucleic acids, in which one strand binds specifically to its exact complement, could not have been designed to be more useful for the study of biology. The ease of designing assays based on this feature is responsible for the incredibly fast pace that has characterized molecular biology research since its inception. With the incorporation of fluorescence technolo...
متن کاملComparative evaluation of commercial fluorescent treponemal antibody-absorbed test kits.
We describe a comparative evaluation of commercial fluorescent treponemal antibody-absorbed test kits, using 150 selected patient sera. The ability of the test kits to detect reactive sera varied from 82.5 to 95%; that for nonreactive sera varied from 80.9 to 96.4%. Reproducibility of reactive and nonreactive results, measured by between-assay and within-assay studies, averaged 42%. The results...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Forensic Science and Technology
سال: 2013
ISSN: 1880-1323,1881-4689
DOI: 10.3408/jafst.18.71